Bioinfomatics and Their Application to Environmental Microorganism
蓄势待发的印度生物技术产业

Methods] Total RNA was isolated from the root of Arabidopsis thaliana, and amplified using RT-PCR methods, After sequencing, the DNA fragment was cloned into the plant binary expression vector containing intron kanamycin gene, and introduced into tobacco varieties by Agrobacterium mediated transformation.Results] The fragment sized 2100 bp was amplied which sequence is the same with AtKup1 gene (Genbank No:AF029876).29 transgenic plants of tobacco were obtained and aproved by PCR, GUS, Southern blot and realtime PCR analysis.The results of chamical content assays confirmed that AtKup1 gene have been expressed effectively in the modified plant and the potassium content in the tabacco leaf have increased 45%.