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中国生物工程杂志 2006
Advances in Study on Lipase Bio-imprinting
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Abstract:
Lipase is a kind of widely used hydrolase. Bio-imprinting is a new technology , it induces the lipase to exhibit exciting catalytic ability in nonaqueous solvents, such as higher activity and stability, and enhances lipase's industrial use as bio-catalysts. The characteristic of the lipase's structure, the process and the mechanism of the bio-imprinting were introduced. Influencing factors such as the selective principle of different kinds of bio-imprinting templates and the function of lyoprotectant through the process of the bio-imprinting were discussed. Meanwhile, the condition of the bio-imprinting, the reaction catalyzed by bio-imprinted lipase and the results were concluded. Finally, the problem and the prospect of the bio-imprinting technology were mentioned.