AOT/Isooctane reverse micellar extraction by adding ethanol Chen Lin-Jie, Cao Xue-Jun
用乙醇消除AOT/异辛烷反胶束体系萃取过程中蛋白质的变性失活

Proteins denaturation or precipitation is a big problem during AOT-isooctane reverse micellar extraction due to strong electrostatic interaction between AOT-proteins molecules. In this paper, it has been resolved by adding ethanol into reverse micellar system, and no denaturation was observed. The phase separation time was shortened significantly. It is only 10 minutes or less to reach phases separation after forward and backward extraction. If this method can be applied in industry, efficiency will be greatly improved. In this study, trypsin was extracted from crude material of pig pancreas by using AOT/isooctane reversed micellar system. Forward and backward extraction recovery of trypsin reached almost 90% and neared 100%, respectively. Finally, about 88% of total yield was obtained, and the specific activity of trypsin was increased to over 1800U/mg with purification factor of 5 times more.