Cloning and Characterization in Structure and Function of Fructan Biosynthesis Enzymes (FBEs) Gene in Plant
植物果聚糖合成酶基因克隆及特性分析

Abstract: Fructans are sucrose-originated polyfructoses, which can be divided into different kinds according to the difference of DP (degree of polymerization) with or without branch structures, the binding type between the two adjacent fructosyls and the different sites of glucoses. Fructans play important roles not only as a source of carbohydrate, but also as agents protecting high plants against abiotic stresses such as water deficit and low temperature. Moreover, they are also utilized as probiotics in human diets, as low-calory sweeteners and are digested in the lower gut, resulting in the decreased incidence of colonic cancer, cardiovascular disease and osteoporosis. Some related studies on fructans including their presence, biosynthesis or degradation pathway, cloning and transformation of encoding genes were presented in this paper. At the same time, we also take in-depth exploration on the copy number, chromosome assignment and sub-celluar localization of fructan biosynthesis enzyme genes (FBEs). The sequence features of FBEs are also analyzed to further clone FBEs genes from more plants, study their function and expression pattern one by one, and improve abiotic stress tolerance and fructan accumulation in some important crops by gene engineering.