The Affection of Molecular Expression in Three-dimensional Cultivating WB-F344 Cells
模拟微重力条件下对WB-F344肝干细胞表征分子表达的影响

To study hepatic stem cells(HSCs) proliferation and characteristic in rotary cell culture system(RCCS) for ideal cell origin of liver tissue engineering and clinic liver diseases stem cells therapy.WB-F344,a kind of rat liver stem cell,was cultured in RCCS to investigate the effect of RCCS onhepatic stem cell characteristics,and the condition of two-dimensional(2D) monolayer cultivating as control.To analyze the changing ofhSCs characteristics by morphological observation,flow cytometry(FACS),RT-PCR and immunofluorescence.To the results,demonstrated that cells were grow well and packed together densely to form irregular aggregates in RCCS,and proliferation of thehepatic stem cells was superior with control,mRNA of AFP increased while ALB decreased,the protein of AFP expression increased while ALB decreased comparing to control.Indicating RCCS could afford appropriate environment to sustain the stem cells character of WB-F344 cells.