Characterization of HA Gene and NA Gene of A pigeon H5N1 Influenza A Virus
鸽源H5N1亚型禽流感病毒株的HA和NA基因特征性分析

RT-PCR were employed to amplify the cDNAs of HA gene and NA gene of A pigeon avian influenza isolate with two pairs of primers. The cDNAs were cloned to pMD18-T and then be sequenced. The results of sequence analysis shows that the cloned HA gene consists of 1707 nucleotides and encodes 568 amino acids residues and contains whole open reading frame. There are 7 potential glycosylation sites and 6 basic amino acids(R-R-R-K-K-R) insert at the cleavage site in HA of the influenza virus isolate which is a symbol of highly pathogenic avian influenza. Amino acids of receptor-binding site are YWIHELY, amino acids of left edge of receptor-binding site are SGVSSA, amino acids of right edge of receptor-binding site are NGQSGR; The cloned NA gene consists of 1350 nucleotides and encodes 446 amino acids residues, there are 3 Potential glycosylation sites in NA of the influenza virus isolate.