Micro-well protein array technology applied in monoclonal antibody isotyping
微孔板蛋白质芯片技术应用于单克隆抗体分型研究

Monoclonal antibody isotyping micro-well protein array was designed and established. Twelve monoclonal antibodies and two polyclonal antibodies were isotyped by the isotyping protein array and ELISA respectively. The results showed that there was no difference between protein array method and ELISA in the application of isotyping monoclonal and polyclonal antibodies. And fewer sample and materials were used and the efficiency was improved in protein array method compared with ELISA. As a result, the antibody isotyping protein array could be used as a sensitive, rapid, directional and economical tool applying in high throughput monoclonal antibody generation.