Preparation of the thiophiolic paramagnetic polymer microsphere and its isolation of the antibody from human serum
嗜硫性顺磁微球的制备及对人血清抗体的分离

The paramagnetic polymer particles with the size range of 0.6–20 μm were synthesized by microsuspension copolymerization with vinyl acetate(VAc) and divinylbenzene(DVB) in the presence of modified magnetite nanoparticles. Evident paramagnetic behavior was observed and saturation magnetization reached 16.7 emu·g 1 at room temperature. These magnetic microspheres were first activated by divinylsulfone, and then modified with mercaptoethanol. By using these thiophilic magnetic microspheres, immunoglobulin G (IgG) could be directly isolated from human serum. This thiophilic magnetic adsorbent performed an evident salt-dependent adsorption behavior for IgG. Due to their strong specificity towards IgG under high salt concentration, the absorbed antibodies could be extracted with high purity. The main factors to influence the isolation, such as the ion types, the ion concentration and the temperature, were also investigated. The technology has great potential to purify antibodies from human serum.