Implication of mating type and cytoplasm factors in asymmetric distribution of two types of monokaryons recovered from dikaryons in Lentinula edodes
交配型因子和细胞质对香菇双核体中两组成型核比例的影响

The genetics background for the phenomenon of significantly distortion to the expected 1 : 1 ratio of the two constitute nucleus in Lentinula edodes was investigated after protoplast monokaryotization.The results reveal that specificity of the B factor,or of a gene linked to the B factor,appeared to influence survival of dedikaryotized nuclei,whereas specificity of the A factor had no apparent effect on this phenomenon:Specificity of B factor correlated with percentage survival of the component nuclear types recovered from both kinds of heterokaryons,it can be put in an hierarchical order with respect to this function,the hierarchical order of these B factors was B1>B3>B4>B2between two tested strains.Data from di-mon mating show that the monokaryons with acceptor nuclear type outnumbered exclusively those entered donors'nuclear types in all secondary derived homocytoplasm heterokaryon,which consists of acceptor cytoplasm,acceptor and donor karyons.It was indicated that some factors in cytoplasm are probably responsible for influencing survival of nuclei recovery from dedikaryotization.Moreover,the nucleus migrated from a dikaryon to a monokaryon is one with stronger compatibility between different B factors.then they fom derived dikaryon.This showed that a pre-entrance nucleus existed in donor and pre-entrance nucleus was mainly dependent on the compatible specificity of B mating-type factor between acceptor and donor nucleus;the higher the specificity,the faster the migration of pre-entrance nucleus.