Cloning and sequencing of a cucumber mosaic virus satellite RNA isolated from Raphanus sativus and comparison of its sequence with 12 satellite
黄瓜花叶病毒萝卜分离株卫星RNA的克隆及其与12个卫星RNA核苷酸序列的比较

The sequence of a sat-RNA of cucumber mosaic virus (CMV-Rs) originally isolated from Raphanus sativus in Hangzhou, Zhejiang Province in April 1998, was determined. This satllite was found consisting of 368 nucleotides (nt). Compared its nucleotide sequence with those of 12 CMV sat-RNAs in document, ranging from 334 nt to 405 nt, resulted three groups and four subgroups. The sequence similarity and grouping were not related to the sizes of the sat-RNAs. The sites of nucleotide insertion or deletion on the 13 sat-RNAs were located at several neighboring sections between 90 nt and 255 nt. The 80 nt at the 5' end, the 45 nt at the 3'end, and unpaired sections in satellite RNA structure were highly conservative. It is thus speculated that a CMV sat-RNA may include 446 nt and could be highly potential for vectoring a foreign fragments for particular purpose.