The lethal and sub-lethal effects of a cry1Ab-transformed rice line KMD1 on Chilo suppressalis (Walker) larvae
转cry1Ab基因水稻对二化螟幼虫的致死和亚致死效应

The lethal and sub-lethal effects of a transgenic rice line KMD1, containing a synthetic cry1Ab gene from Bacillus thuringiensis measured on larvae of striped stem borer, Chilo suppressalis (Walker) is reported in this article. The transgenic rice line was highly toxic to the 1st, 3rd and 5th instar larvae. When larvae of the three instar groups tested continually fed on the transgenic rice stems, all of them ultimately died and failed to pupate, even though the 5th instar larva were able to survive for a relatively long time. In contrast, those larvae which fed on untransformed control rice stems after feeding on transgenic tissues for a period time had a lower mortality and was able to survive into the next instar and, partly, even to the pupal stage, but there was no significant difference in development time between treated larvae and the control. The larvae of the tested instar groups, except the 1st instar, when reared with the control tissues after feeding on the transgenic tissues, Succeeded in pupa formation followed by emergence. Their emergence percentage and egg laying performance were not inferior to the control. As regard to body weight and body length of moths developed from the treated larvae, no significant difference from the control was observed provided the larvae were treated since the 3rd instar, whereas significantly limited body weight and length of moths resulted if the treatment was performed since the 5th instar.