Study on the effect of plant hormones and pre-chilled treatment to break dormancy and germination of Rhodiola rosea seeds
植物激素调节和预先冷处理对破除红景天种子休眠和发芽的影响

Rhodiola rosea has been widely used as traditional herb medicine in China for centuries. Its seeds germinate unevenly and at a low rate; this is due to dormancy of the seed. We use pre-chill treatment and exogenous plant hormones stimulation to break dormancy of Rhodiola rosea seeds, to trigger seed germination and to gain high quality seedlings; as well as to research the physiologicalinteractions between exogenous stimulation, endogenous plant hormone change, the germination of seedsand growth of seedling. It is observed that seeds pre-chilled at 4 ℃ for three weeks should the highestgermination rate at 25-10 ℃, the rates of germination get to (48± 2)%. From our research, we foundthat in the embryos of seeds of Rhodiola rosea the endogenous level of ABA decreased and thenmaintained at low level after being treated with exogenous GA3, 2,4-D and GA3 +2,4-D. Medium levelof endogenous IAA (from 611.8 ng/gFW to 494.6 ng/gFW) can balance the growth of root and shoot,endogenous IAA content change at this range, the survival rate of seedling is the highest.