Cloning of the promoter sequence of rice OsPR-4b gene and construction of its 5'''' deletions for analyzing promoter activity
水稻病程相关蛋白基因OsPR-4b启动子的克隆及缺失体构建

A 2257 bp promoter sequence upstream of the rice OsPR-4b was amplified using two primers designed on the basis of the sequence of OsPR-4b and rice genome sequences database. Several cis-acting elements, including W-box, PAL-boxA, GT-1 elements and MybSt1 elements, were recognized with the aid of PLACE program. The promoter region sequence and its 5' deletion constructs were fused to the gus reportor gene in pCAMBIA1391z vector for analyzing the promoter activity of the cloned promoter sequence and the functions of the cis-acting elements in regulation of OsPR-4b expression.