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OALib Journal期刊
ISSN: 2333-9721
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Rapid detection of specific proteins expressed in cytoplasmic male-sterile line of tuber mustard using SDS-PAGE
利用SDS-PAGE电泳快速检测茎用芥菜细胞质雄性不育系特异表达蛋白(英文)

Keywords: tuber mustard,organ-specific proteins,cytoplasmic male-sterility
茎用芥菜
,芥菜,器官特异性蛋白,细胞质雄性不育,SDS-PACE电永

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Abstract:

In this paper, two specific proteins with molecular weights of ca.14.4 kD and 43.0 kD were detected by SDS-PAGE in floral organs of CMS line of tuber mustard , of which the 14.4 kD protein was clearly over-expressed. Nevertheless, no difference was detectable in banding patterns of soluble proteins of leaves and roots between CMS and male fertile lines during both vegetative and reproductive stages. It is suggested that the expression of specific proteins is quite organ-specific and over-expression of the 14.4 kD protein may be responsible for CMS of tuber mustard .

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