Efficient Production of Doubled Haploid Plants through Microspore Culture in Brassica napus
油菜小孢子培养以有效产生纯合两倍体植株

Since haploid embryoids were first obtained from microspore culture of Brassica napus there has been a rapid progress in the application of this technique in genetic studies and breeding utilization of rapeseed.The relative simplicity of the culture protocol and high-frequency recovery of doubled haploid plants makes microspore culture a potentially powerful technique for varietal development.Nevertheless, there are still some obstacles in the efficient use of this technique such as recalcitrant genotypes, secondary embryogenesis of embryoids, and the lack of efficient chromosome doubling.The present experiment is attempted to improve the production efficiency of doubled haploid rapeseed plants.