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浙江大学学报(农业与生命科学版) 2001
Comparison on leaf ultrastructure in cytoplasmic male sterile line for tuber mustard (Brassica junceavar. tumida ).
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Abstract:
Ultrastructure of leaf was compared between cytoplasmic male sterile line (CMS) and its maintainer line(MF) during the development of tuber mustard. It was indicated that: Chlorophyll content in leaves of CMS was deficiency than that of MF. Difference was also observed in cell ultrastrucature between CMS and MF. Chloroplast of CMS' leaves was under developed than that MF in rosette, which was ellopsographical shape. Also, the number of stacks in each granum was reduced .While little starch was acculmated in CMS. The activity of mitochondria was also lower with little intracristaed membrane. Furthermore, almost no endoplasmic reticulum was detected in CMS. But, on swelling stage, the developed level of chloroplast structure in CMS was as near as that in MF, which became spear shaped. Likewise, the lamella's system was in well developed and clear state. The corresponding activity of mitochondria was also enhanced obviously. However, when to peduncle growing period, both of CMS and MF's ultrastructure became retrogressive, intracristaed membrane of organelle was degraded and disappear, while the activity of mitochondria was also decreased, a lot of starch was acculmated in the chloroplast of CMS and MF. Based on the results, the relationships between cytoplasmic male sterility and ultrastructure were also discussed.