Expression and analysis of partial functional domains of H11 gene from Haemonchus contortus
捻转血矛线虫H11部分功能域基因的原核表达与分析

Partial fragment of H11 gene containing several functional domains from Haemonchus contortus was cloned and expressed after bioinformatics analysis of H11 with the soft ware.The fragment of H11 gene from 670 bp to 1710 bp was amplified by means of polymerase chain reaction(PCR) with a pair of primers which was designed according to the published data of our laboratory on GenBank and was named HPS(H11 partial sequence).Then the HPS gene was ligated to the prokaryotic expression vector pET-28b and tansformated into E.coli BL21(DE3).The positive clones were verified by sequencing.Sequence analysis shows that the gene obtained shared 100% and 98.8% identity to the published full coding sequence of H11 gene from Haemonchus contortus ZJ strain and the reference strain,respectively.And the positive clone was induced by IPTG.The results indicate that the fusion protein was calculated to be about 37.34 kDa by SDS-PAGE.The expression quantity of the recombinant fusion protein could reach 58.9% after being induced for 6 h.Western-blotting analysis with anti-his antibodies against the fusion protein showed the recombinant protein shared specificity.