Biological activity of Piper sarmentosum Roxb petroleum ether extractand its effects on metabolic enzymes of Aleurodicus disperses Russell
假蒟石油醚萃取物对螺旋粉虱的生物活性及代谢酶活性的影响

Aleurodicus disperses Russell is a dangerous new invasive pest in Hainan Province with serious harm. This study was conducted to explore the biological efficacy of petroleum ether extracts from Piper sarmentosum Roxb on A. disperses. Petroleum ether extracts from P. sarmentosum were used at different concentrations to investigate their effects on A. disperses adults, nymphs and pupae using the leaf immersion method. Also the activities of AChE, CarE and GSTs in adult A. disperses were investigated via biochemical methods. The results indicated that petroleum ether extracts from P. sarmentosum had high bioactivities on adult, nymph and pupae A. disperses, which increased with lapse of time. LC50 value after the petroleum ether extracts application on nymph A. disperses for 168 h (0.932 mg·mL-1) was much lower than that for 24 h of treatment (2.207 mg·mL-1). When treated for 72 h, LC50 values of P. sarmentosum petroleum ether extracts application on A. disperses adults and nymphs and pupae were 0.430 mg·mL-1, 1.303 mg·mL-1 and 1.894 mg·mL-1, respectively. When treated for 24 h, AChE, CarE and GSTs in adult A. disperses were to some degree restrained. The inhibition rates of 10 mg·mL-1 concentration of P. sarmentosum petroleum ether extracts on AChE, CarE and GSTs of A. disperses adults were 82.53%, 82.79% and 52.26%, respectively. Whereas the activities of CarE and GSTs increased with decreasing dose of P. sarmentosum petroleum ether extracts, that on GSTs was a lot more obvious. In conclusion therefore, P. sarmentosum petroleum ether extract toxicity intensively inhibited A. disperses pest invasion in Hainan Province.