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Study on genetic relationship among 4 kinds of abalone using RAPD technique
应用RAPD技术研究4种鲍的亲缘关系

Keywords: Haliotis discus discus,H,discus hannai,H,diversicolor diversicolor,H,diversicolor supertexta,Random amplified polymorphic DNA (RAPD),Genetic relationship
盘鲍
,皱纹盘鲍,杂色鲍,九孔鲍,RAPD,亲缘关系

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Abstract:

RAPD technique is used to study the genetic relationship among 4 kinds of abalone. The results show that total 538 DNA bands are scoped by 20 effective primers in 4 kinds of abalone, of which 136 DNA bands are reproducibly polymorphic, and the percentage of polymorphic products is 25.3%; the genetic distance and genetic identity between Haliotis discus discusand H.discus hannai are 0.28, 0.72 respectively, those between H.diversicolor diversicolorand H.diversicolor supertextaare 0.32, 0.68 respectively. In the results of cluster analysis, H.discus discusand H.discus hannaiare pooled into one group, they both are genetically closer; H.diversicolor diversicolorand H.diversicolor supertextaare pooled into another group, they both also are genetically closer.

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