Cloning and expression analysis of PaAGAP gene from Phytolacca acinosa in response to various kinds of stress
商陆小G蛋白激活蛋白基因PaAGAP在逆境下表达研究

A full-length cDNA of an ArfGAP ( PaAGAP ) encoding 332 amino acids was cloned from Phytolacca acinosa by using methods of homology-based cloning and RACE. PaAGAP is predicted to contain a CX2CX16CX2C type zinc finger domain and a C2 domain. Real-time PCR analysis revealed that the expression of PaAGAP was up-regulated in response to low temperature, osmotic, salt, and heavy metal stresses, indicating a potential role of PaAGAP in the plant stress response that involves the inactivation of ARF and the inhibition of polar auxin transport.