|
中国科学 生命科学 2010
Different forms of nitrogen utilization and effect of exogenous glucose on their metabolism in germinating spores of AM fungus
|
Abstract:
Nitrogen utilization and amino acids biosynthesis were examined in germinating spores of AM fungus Glomus intraradices Schenck & Smith after exposure to various N substrates,glucose and/or root exudates.N uptake and de novo amino acids biosynthesis were analyzed using stable isotopic labeling with mass spectrometric detection.HPLC-based analysis was used to measure amino acid levels.In the absence of exogenous nitrogen sources,germinating AM fungal spores utilized internal storage N compounds for biosynthes...