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OALib Journal期刊
ISSN: 2333-9721
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Comparative Analysis of Oryza latifolia Genome with C0t-1 DNA and Genomic DNA of Oryza sativa
利用栽培稻C_0t-1 DNA和基因组DNA比较分析宽叶野生稻基因组

Keywords: C0t-1 DNA,Genomic DNA,FISH,Stringency,Oryza latifolia
C0t-1DNA
,基因组DNA,荧光原位杂交,洗脱严谨度,宽叶野生稻

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Abstract:

C0t-1 DNA and genomic DNA(gDNA) of cultivated rice Oryza sativa were used as probes to analyze comparatively the Oryza latifolia genome by fluorescence in situ hybridization(FISH).Results showed that the hybridization signals of gDNA were more distinct on the chromosomes of O.latifolia than that of C0t-1 DNA.These hybridization signals were mostly distributed on centromeres and telomeres regions as well as those near centromeres under different stringencies.With increasing washing stringency,hybridization s...

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