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植物科学学报 2011
Virus-resistance of Papaya Ringspot Virus Mediated by Double-Strand RNA
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Abstract:
Tobacco(Nicotiana tabacum),Arabidopsis thaliana,and host papaya(Carica papaya L.)of PRSV were used as model plant materials to testing whether RNAi could mediate resistance to papaya ringspot virus(PRSV).The agrobacterium was harbored with pHellsgate12-CPIR containing structured inverted sequences of the CP gene of PRSV,where were transformed into the tobacco and Arabidopsis.We also conducted an Agrobacterium-mediated transient expression experiment by infiltration in papaya.The three transgenic plants inoc...