Analysis of Methylation at CpG Island of FIE in Rice by Bisulfite Sequencing
亚硫酸氢钠测序法检测水稻FIE基因CpG岛甲基化状态

To test the feasibility of bisulfite sequencing in methylation analysis at a specific gene of rice,we analyzed DNA methylation at fragment of CpG island of FIE2A.In the present paper,genomic DNA was extracted from leaf and endosperm of rice with the CTAB method.After treated with bisulfite sodium,we amplified the CpG island of FIE gene with designed primers using nest-PCR,sequencing results after cloning in TA-vector.Results showed that nest-PCR increased the specificity of the PCR products,and symmetric se...