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植物科学学报 2011
RAPD Analysis with Random Primer Combinations and SCAR Identification of Gynostemma pentaphyllum
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Abstract:
The aim of this study was to develope species-specific molecular marker for identification of Gynostemma pentaphyllum and that with their adulterant Cayratia japonica,in order to avoid adulteration that affects the quality and quantity of G.pentaphyllum The first,twenty 10-bp random primers were picked out from more than 2000 random primers and then divided randomly into four terms that were consist of five random primers.The second,Genomic DNAs of seven G.pentaphyllum from different places in Guangxi,China...