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OALib Journal期刊
ISSN: 2333-9721
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CONSTRUCTION OF EXPRESSION VECTORS FOR CEREAL MALE STERILITY AND ITSFERTILITY RESTORATION
禾谷类作物雄性不育及育性恢复表达载体的构建

Keywords: Male sterility,Fertility restoration,Expression vector,Cereal
雄性不育
,育性恢复,表达载体,禾谷类作物

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Abstract:

The tapetum- promoter( CA55) was amplified from the total DNA of Zea mays L.by polymerase chain reaction,and was fused with barnase( BN) and barstar( BS) genes,respectively.The male sterile gene.CA55BNNOS and its fertility restoration gene CA55BSNOS were construced.They were cloned into p CAMBIA330 0 ,respectively.The expression vectors for cereal male sterility and its fertility restoration were obtained.

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