Pre-Pressing Treatment for Cissus quadrangularis L. and Other Small Succulents
Cissus quadrangularis L.及其它小型肉质植物标本制作中的压制前处理

Herbarium specimens of Cissus quadrangularis L. were prepared by different techniques and compared for shrinkage, retention of color, and persi- stence of stem appendages. The efficacy of three pretreatment techniques and two methods of handling succulent tissue were investigated. Using analysis of variance (ANOVA) there was shown to be a significant difference (P<0.005) between the three pretreatment techniques. Using the least significant diffe- rence(LSD) test, the deep- freezing technique was shown to produce less distortion in the resultant specimens than the gasoline technique (LSD=1.708) and the formalin technique (LSD=2.065). No significant difference was observed in the quality of specimens produced by the formalin and the gasoline techniques (LSD=0.357NS). ANOVA test indicated a significant difference in efficacy between the acetic acid treatment, the longitudinal sectioning treatment and untreated specimens (P<0.001). The acetic acid pretreatment was shown to be superior to the longitudinal sectioning pretreatment (LSD=2.371) and untreated specimens (LSD=2.138). No significant difference was found between the untreated specimens and those subjected to longitudinal sectioning (LSD=0.233NS). The acetic acid pretreatment technique is recommended for treating specimens of small succulents by herbarium workers.