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植物科学学报 2004
The Cytomorphology Study of Anther Abortion in Male-Sterile Lines of Hong Cai-tai (Brassica campestris L.ssp.chinensis L.var.utilis Tsen et Lee.)
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Abstract:
Microsporogenesis of Hong Cai-tai's Polima cytoplasmic male-sterile line (Polima CMS),Hong Cai-tai's Ogura cytoplasmic male-sterile line(Ogura CMS) and their maintainers were investigated cytologically by using paraffin section with light microscopy systemly.The observation showed that the anther development of Hong Cai-tai's Polima male-sterile line was blocked without anther at the stage of archesporial cell.The few pollen and no anther cell was observed without forming the tapetum and the middle layer;while the anther development of Hong Cai-tai's Ogura male-sterile line was blocked without anther at the stage of microspore mother cell & tetrads of microspores,observing the tapetum cell abnormal and tetrads of microspores being extruded.In the end the tapetum cell and tetrads of microspores disaggregated.