Progress of Plant FISH Technique and Its Applications in the Analysis of Plant Genome
植物荧光原位杂交技术的发展及其在植物基因组分析中的应用

Fluorescence in situ hybridization(FISH) is an effective and accurate molecular cytogenetic tool for mapping specific DNA sequences on chromosomes,interphase nuclei and DNA fibers.Rapid advances in plant FISH technique have been made in the past two decades.Dual FISH,multicolor FISH and multiprobe FISH cocktail have been developed to increase the number of detected targets;BAC-FISH and tyramide signal amplification FISH(TSA-FISH) have been applied to increase the detection(sensiti-)(vity) of very small chromosomal targets;and high resolution FISH such as pachytene FISH,interphase FISH,fiber FISH and FISH on super-stretched flow-sorted plant chromosome have been developed mainly to improve the spatial resolution of signals derived from flanking sequences.FISH have played important roles in the analysis of plant genome.It can physically map specific DNA sequences,provide effective marks for chromosome identification within a genome,investigate the evolution of plant genome by comparative mapping of the same DNA sequences in related species,construct the physical map of plant genome,directly reveals the DNA molecular organization of a specific chromosome regions,analyze the organization pattern of interphase chromatin and the dynamic changes of chromosomes in cell cycle,and characterize transgenes in plant genomes.