Factors Affecting in vitro Shoot Elongation of Pepper
影响辣椒离体再生芽伸长因素研究

A detailed study was carried out to investigate key factors affecting shoot elongation of pepper. Using the cotyledons of pepper as explants, the optimal phytohormone combination of BA+IAA for bud induction was screened out to investigate the effect of GA3, bud-inducting time, organic constituents in media, different phytohormone combinations and cultivars on shoot elongation. The results were as follows:Seven cultivars showed ability differences for bud induction,and BA 3.0 mg·L-1+IAA 0.5 mg·L-1 could give the highest response. The shoot elongation frequency increased with GA3 concentration, with the optimal concentration of 1.0-2.0 mg·L-1. The buds subjected to 21-day induction showed higher elongation efficiency than that of 14-day induction.B5 vitamins were more efficient than MS vitamins for shoot elongation. The phytohormone combination of Zeatin+GA3 exhibited higher elongation efficiency than combinations of BA+IAA+GA3 or BA+PAA+GA3.Elongation ability varied with pepper cultivars, with Chufeng and Sujiao No.5 having the best elongation ability. Compared with IAA and NAA, IBA gave better rooting effect.