Preliminary Studies on the Isolation of Microsatellite Loci of Manglietia patungensis (Magnoliaceae)
巴东木莲核基因组微卫星位点分离的初步研究

FIASCO is a high-efficiency method for constructing microsatellite-enriched genomic library.A microsatellite-enriched genomic library of Manglietia patungensis was constructed by using the method of fast isolation by AFLP sequences containing repeats (FIASCO).The microsatellite sequences and their characteristics were analyzed in this study.One hundred and nineteen clones which were 400-500 bp in size were isolated and sequenced from Manglietia patungensis microsatellite libraries.Fifty seven clones of them...