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植物科学学报 2010
rDNA ITS Sequence Analysis of Different Cultivar Variety of Cornus officinalis
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Abstract:
To study rDNA ITS sequence of Cornus officinalis Sieb.et.Zucc.,and analyzed the rDNA ITS sequence of its different cultivated varieties.A pair of primers was selected by experiment.The ITS gene fragment was amplified using this pair of primers,and PCR products were purified with DNA gel extraction kit(AxyPrepTM)and sequenced by the method of Sanger dideoxy.Then the ITS1-5.8S-ITS2 sequences were analyzed by the software of DNAssist Version 2.0.The ITS1-5.8S-ITS2 sequences was found whole ITS sequence consist...
