Analysis of Non-gel Sieving Capillary Electrophoresis in Protein Changes Pattern in Development Phase Shift in Apple
无胶筛分毛细管电泳在分析苹果发育时期转变蛋白质变化规律中的应用

Protein markers were used with optimal electrophoresis conditions in analysis of proteins of phase change in apple.Optimal conditions for non-gel sieving capillary electrophoresis (NGSCE) were 0.15 mol.L-1 TB, 0.1% SDS, 7.5% Dex tran, 7.5% glycerol, 23.5 kV separation voltage and 1.5 psi×90 s injection volume. This condition for NGSCE was used to separate the phloem proteins in apple hybrid seedlings. A 5.0-kDa protein appeared above the 50th node, and the content of a 24.5-kDa protein increased sharply during phase change. The result was valuable for study of apple phase change.