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植物学报 2006
Tissue Culture and Plant Regeneration from Immature Inflorescence Explants of Suaeda salsa
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Abstract:
A protocol for high frequency adventitious shoot induction and plantlet regeneration from immature inflorescence explants of Suaeda salsa (a C3 halophyte) was reported. Adventitious shoots were induced from immature inflorescence cultured on Murashige and Skoog (MS) medium supplemented with 1.0 mg.L-1 6-benzylaminopurine (6-BA) and 0.4 mg.L-1 indole-3-acetic acid (IAA) for 25 days. Induction frequency of adventitious shoots was about 82.1%. Adventitious shoots were further multiplied vigorously and maintained for a long time on the same medium for adventitious shoot induction. Rooting was achieved on MS medium without any plant growth regulators after adventitious shoot was transplanted for 2-3 weeks.