Chinese tomato yellow leaf curl virus--a new species of geminivirus

Chine tomato yellow leaf curl virus (TYLCV-CHI) and other geminiviruses were analysed with 20 monoclonaI antibodies. It was shown that TYLCV-CHI is serclogicaIly close to Chinese tabacco Ieaf curl virus (TbLCV-CHI). The fragment of TYLCV-CHI DNA including the common region (CR), N-terminal of coat protein gene and AV1 gene was amplified by PCR and cloned, and its DNA sequence was determined. These raults showed that TYLCV-CHI is different from other known geminiviruses in the world, and is a new whitefly-transmitted gerninivirus. Project supported by tile National Natural Science Foundation of China (Grant No. 39470034) and Chins-Israel Fund for Scientific and Strategic Reserch and Development. The nucleotide sequence data reported in this paper will appear in the DDBJ, EMBL and Genebank nucleotide sequence databases with the accession number D88773.