Sequencing and bacterial expression of a novel murine alpha interferon gene

A murine new alpha interferon gene (mIFN-αB) was found by primer-based sequencing method in a murine genomic DNA library. The gene was cloned and its sequence was determined. It was expressed inEscherichia coli under the control of the PL promoter which resulted in antiviral activity on mouse L-cells. The sequence of mIFN-αB has been accepted by GENEBANK.