Cloning and Expression of Rubisco Activase Gene in Wheat
小麦Rubisco 活化酶基因的克隆和表达特性

Rubisco activase is an ubiquitous enzyme for the activation of Rubisco in photosynthetic autotrophs. A cDNA fragment of Rubisco activase gene was cloned from wheat (Triticum aestivum). Northern blot showed that expression of the gene was down-regulated in dark-induced senescence leaves, where photosynthetic rate, chlorophyll content and Rubisco activity also showed obvious decline. The results suggest that the decreased expression level of the gene was related to the decline in photosynthetic rate.