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植物学报 2008
Agarose Blotting: a Simple Way to Observe Plant Epidermal Cells
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Abstract:
We describe a new method, agarose blotting, used to detect epidermal cell size and number of plant organs. With this method, clear images of epidermal cell shape can be observed through several steps of fixation, agarose embedding, and agarose cutting by using the plasticity of agarose. This method is easy to use, saves time, is widely applicable and produces accurate results with a clear picture, in compiling statistics of cell size and number during the process of plant development.