Study on the Methods of Extraction of Protein from Paulownia Leaves
泡桐叶片蛋白质提取方法的研究

The methods of extraction of protein from Paulownia leaf were studied in this paper. The results indicated that the amount and molecular weight of the protein extracted with UKC (9.5 mol/L urea, 5 mmol/L K2CO3, 1.25% cetyltrimethylammonium bromide, 0.5% dithiothreitol, 2% ampholine pH3.5 - 10,5% Triton X-100) from Paulownia leaf treated with cool (- 40 C ) TCA (contained 0.07% 2-mercaptoethanol) were the most and largest respectively among 24 combinations of protein extraction methods. This implied that the method might be the best one of extracting protein from Paulownia leaf.