Isolation and mapping of bacterial artificial chromosome (BAC) clone containing telomere-associated sequence

Using single primer (TTTAGGG)\-3 or (CCCTAAA)\-3CCC corresponding to rice telomeric repeat sequences, two fragments were amplified from rice genomic DNA and named Tas1 and Tas2. These two fragments did not show polymorphism between the mapping parents. Tas1 was found to hybridize to rice telomeric regions by in situ hybridization (FISH). Then a rice bacterial artificial chromosome (BAC) library was constructed and Tas1 was used to screen it. One positive clone was identified. A single copy sequence of this BAC clone was mapped at the end of the 6th chromosome of rice.