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植物学报 2005
Effects of Various Factors on the Growth and Nicotine Production of Hairy Roots of Nicotiana tabacum in Suspension Culture
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Abstract:
悬浮培养中烟草(Nicotiana tabacum)发状根的生长及烟碱合成受到基本培养基浓度、初始pH值、激素种类和浓度等因素的影响。一个5 cm长的根尖, 悬浮在40 mL、pH值为6.0、附加1.0 mg.L-1IAA的1/2 MS液体培养基中, 28 ℃、散光条件下培养30天, 烟碱产量可达0.241 mg.mL-1。