Effects of water stress on protein expression and physiological properties of different genotype wheat(Triticum aestivum L.)sprouts
水分胁迫对不同基因型小麦幼芽蛋白质表达和某些生理特性的影响

With drought-resistant wheat(Triticum aestivum)cuhivar Changwu 134 and droughtsensitive cuhivar Zhengyin 1 as test materials,and by using-1.2 MPa PEG 6000 to treat their seeds,this paper studied the protein expression and physiological properties of the sprouts under different soil moisture conditions.SDS-PAGE analysis showed that water stress induced the production of two new proteins with molecular weights of 39.5 kDa and 23.0 kDa in Changwu 134 but not in Zhengyin 1 sprouts.Under normal water supply,the expression of the protein with molecular weight of 48.5 kDa in the sprouts of both Changwu 134 and Zhengyin 1 increased with sprout growth.This protein was preliminarily named as water-sensitive protein,due to lts wate-sensitivity and of newly discovered protein.The determinations of physiological properties showed that under water stress,the sprouts of drought-resistant Changwu 134 had higher root/shoot ratio and higher relative water content,but lower relative membrane permeability and lower malondlaldehyde content than those of drought-sensitive Zhengyin 1.