Random amplified polymorphic DNA identification of six Bactrocera (Diptera:Tephritidae) species in Yunnan Province of Southwest China.
云南六种实蝇的RAPD快速鉴定

In this paper,the genetic relationships among six fruit fly species of Bactrocera in Yunnan Province of Southwest China,i.e.,B.tau,B.scutellaris,B.scutellata,B.cucurbitae,B.dorsalis and B.correcta,were studied by using the technique of random amplified polymorphic DNA(RAPD).Five primers with good repetition and high polymorphism were screened from 131 primers,and a total of 302 bands were amplified,among which,111 bands were of genetic polymorphism.The primers OPC-01,OPI-17,OPL-07,OPL-08 and OPL-16 could be used for the identification of the 6 fruit fly species.