Isolation and identification of a phenanthrene-degrading bacterial strain
一株菲降解细菌的分离鉴定及其特性

Through selective enriched culture, a phenanthrene-degrading bacterial strain was isolated from the oil-contaminated soil in Shenfu irrigation area of Shenyang, Northeast China. The morphological and physiological-biochemical identification, 16S rDNA sequence analysis, and phylogenetic study showed that this strain was belonged to genus Acinetobacter and named as Acinetobacter sp, L2, and closest to Acinetobacter sp. DG880 AY258108 ]. It could use phenanthrene as the sole carbon source, Mter 7 days culture, the degradation rate of phenanthrene was 96. 3%. According to the activity of catechol 2,3-dioxygenase, the strain probably had phenanthrene-degrading genes.