Application of RAPD in identifying Phellinus baumii strains
随机扩增多态性DNA技术在鲍氏层孔菌菌株鉴别中的应用

In this paper, random amplified polymorphic DNA (RAPD) analysis was conducted with 20 random primers in 7 strains of Phellinus baumii collected from different localities. The results showed that 17 of the 20 random primers were polymorphic ones. The DNA bands derived from each primer amplifying in tested strains ranged from 10 to 33. The size of the amplified DNA fragments ranged from 250 to 2000 base pairs. Of each test primer, a wide variation in banding profiles was observed among the 7 strains of P. baumii. A total of 377 band positions were scored for all of the test strains, which differed significantly among the bands from different primers. UPGMA cluster analysis subdivided the test strains into two groups, which was helpful to find out the difference among the test strains and to distinguish them directly.