Effects of amino acid-N and ammonium-N on wheat seedlings under sterile culture
无菌条件下小麦氨基酸态氮及铵态氮营养效应研究

The dry weight, total N, and glutamate-oxaloacetate transaminase(GOT) and glutamate-pyruvate transaminase (GPT) activities in roots and leaves of wheat seedlings (Triticum aestivum) grown with ammonium sulfate or amino acids (glycine, glutamate or lysine) were studied under sterile sand culture. The results showed that both NH4(+)-N and amino acid-N could be absorbed by wheat. The total N of plant fed with NH4(+)-N was similar to that fed with amino acid-N. The dry weight of plants grown 30 days with glycine or glutamate was significantly higher than that of plants grown with NH4(+)-N or free N. The dry weight of ammonium treatment was similar to that of lysine treatment or free N. NH4(+)-N in concentration of 0.7 mmol.L-1 significantly increased GPT activity of roots, but had no significant effects on leaves or roots treated 6 h in concentration of 35.7 mmol.L-1. Different species or concentrations of amino acids had different abilities to increase the GOT or GPT activity in leaves or roots.