Identification of Markers Linked to Resistance Locus of Marssonina Leaf Spot in Poplars by Bulked Segregant Analysis(BSA)
美洲黑杨抗黑斑病基因的RAPD标记筛选和连锁分析

DNA markers linked to resistance locus of Marssonina leaf spot in poplars were found by bulked segregant analysis (BS A). The bulks consisted of individual with a extreme phenotype taken from a population of 91 Fj clones, which is a progeny of Populus deltoides Bartr. cv. "Lux"(I-69/55) (Resistance) and P. euramericana cv. I-45(Susceptible). Out of 114 RAPD primers,four markers showed polymorphisms between the resistance-bulk and the susceptible-bulk. By using selective genotype linkage analysis, OP All 7 -1550 and OPAI13 - 900 were found linked to the resistance locus. The genetic distances between the two markers and the resistance locus were 29. 9cM and 37. 4cM,respectively.