PCR-RFLP Analysis on Roegneria, Elymus, Hystrix and Kengyilia in Triticeae (Poaceae)
小麦族鹅观草属、披碱草属、猬草属和仲彬草属细胞质基因组PCR-RFLP分析

PCR-RFLP of Plasmon on 23 species of Roegneria,Elymus,Hystrix and Kengyilia and 1 outgroup(Triticum aestivum L.) was carried out.The segments amplified with 3 cpDNA and 3 mtDNA universal primers were digested by 15 restriction endonucleases.Among 329 bands produced,304 were polymorphic(92.4%).PCR-RFLP results showed that polymorphisms existed between the four genera and between the species in Roegneria,Elymus,Hystrix and Kengyilia,and genetic similarity coefficient was high.Roegneria grandis,R.aristiglumis,R.elytrigioides,R.alashanica and R.magnicaespes were grouped,while R.caucasica,R.ciliaris,R.amurensis and R.japonensis clustered together.Species of Kengyilia clustered independently.Five species of Elymus,3 of Hystrix and Roegneria kamoji grouped together.The results suggested Kengyilia was a valid genus.As more cytological and molecular data are available,the taxonomic status of Roegneria,Elymus and Hystrix will be recognized.