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ISSN: 2333-9721
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遗传  2000 

An Improved Method for Increasing the Efficiency of the Technique of Random Amplified Polymorphic DNA(RAPD)
一种提高RAPD技术扩增效率的有效方法 An Improved Method for Increasing the Efficiency of the Technique of Random Amplified Polymorphic DNA(RAPD)

Keywords: RAPD,spidle,ramp,efficiency,finger printing
RAPD
,蜘蛛,ramp,效率,指纹

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Abstract:

The random amplified polymorphic DNA(RAPD)is a technique of DNA molecular marker,which was first established by Williams in 1990.The resolution and repetition is low because of the low annealed tempture and short primers in the RAPD.In this paper we introduce an improved method for increasing the efficiency of the technique of RAPD by prolonging the ramp time from annealing to extension and increasing the resolution and production.

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