Studies of DNA Polymorphism at D7S21 Locus in Hebei Han Population
用改进的MVR-PCR方法检测河北地区汉族人群D7S21基因座多态性 Studies of DNA Polymorphism at D7S21 Locus in Hebei Han Population

To study the polymorphism at D7S21 locus in Hebei Han population, 124 unrelated individuals were detected rapidly by Minisatellite Variant Repeat-Polymerase Chain Reaction (MVR-PCR) and polyacrylamide gradient gel electrophoresis followed by silver staining,and digital codes were obtained. About 36 digital codes were obtained from each individual. No two unrelated individuals shared the same codes. The probability of identity in 36 digital codes was 3.48 x 10(-18).The percentage of three repeat units, a-type, t-type and 0-type was 48.5%, 49.4% and 2.1% respectively. The heterozygosity (H), excluding probability of paternity (EPP)and polymorphism information content (PIC) were 0.9876, 0.9746 and 0.9872 respectively. The results suggested that D7S21 locus has highly polymorphism in Hebei Han population.The method-polyacrylamide gradient gel electrophoresis followed by silver staining was simple,rapid and practical.